FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71432
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT350.0295.000031
TTACCCT350.0295.000031
GCCCCTA900.0295.02
TCGCCCT550.0295.01
GCGACCT255.2568794E-10295.01
GATCCTA1500.0295.02
GACCCCT157.2120147E-6295.01
GTAGCCT157.2120147E-6295.01
TCTACGG157.2120147E-6295.04
CGAGCCT450.0295.01
CGTCCTA1000.0295.02
CGATCCT303.6379788E-12295.01
ATCGCCT303.6379788E-12295.01
GCATCCT303.6379788E-12295.01
GTGCCTA303.6379788E-12295.02
TGATCCT800.0294.999971
GTCGCCT108.448439E-4294.999971
CGCGAGG108.448439E-4294.999979
CGTTCCT206.144546E-8294.999971
ACGTCCT206.144546E-8294.999971
Produced by FastQC (version 0.11.5)