FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79198
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT450.0295.01
GCGCGAA108.4501697E-4295.0295
GATCCTA1450.0295.02
CCTACGC157.2149705E-6295.05
CGCGAGG108.4501697E-4295.09
TACGGGC108.4501697E-4295.07
TACGCCT255.275069E-10295.01
CGGGACA108.4501697E-4295.09
CGGTCCT450.0295.01
CGGTAGG108.4501697E-4295.09
TTCGCCT255.275069E-10295.01
CGATCCT450.0295.01
TCGACCT450.0295.01
GTGCCTA650.0295.02
GTCCTAC4800.0291.92713
TCCTACG22500.0289.755554
CCTACGG75200.0288.527255
TACGGGA71750.0288.42167
CTACGGG73450.0287.569766
GCCTACG12850.0286.9654
Produced by FastQC (version 0.11.5)