FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54154
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22684.188056283931012No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22384.132658714037745No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22204.099420172101784No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA21984.058795287513388No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17733.2739963806921004No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17163.168740997894892No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16002.9545370609742587No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15922.939764375669387No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15912.9379177900062783No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15892.9342246186800605No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15872.9305314473538426No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15842.924991690364516No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15802.9176053477120805No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15622.8843668057761196No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14062.59629944233113No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13142.4264135613251097No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13122.422720389998892No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12952.3913284337260405No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3400.6278391254570299No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3220.5946005835210696No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3090.5705949699006537No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2890.5336632566384755No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2750.5078110573549507No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2540.4690327584296636No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.46533958710344575No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2410.44502714480924765No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2380.439487387819921No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2350.43394763083059423No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2340.4321010451674853No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2240.4136351885363962No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2240.4136351885363962No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2220.40994201721017837No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.4007090888946338No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2150.397015917568416No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2150.397015917568416No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2130.3933227462421982No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2130.3933227462421982No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2080.38408981792665364No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2010.37116371828489125No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2000.36931713262178234No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1920.35454444731691104No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1820.3360785906858219No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1820.3360785906858219No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.334232005022713No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1790.33053883369649517No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1790.33053883369649517No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1770.3268456623702774No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1670.3083798057391883No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1650.3046866344129704No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1620.2991468774236437No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1600.2954537060974259No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1600.2954537060974259No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1560.28806736344499023No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1520.2806810207925546No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1510.27883443512944567No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.2696015068139011No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.26406174982457437No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1420.26221516416146545No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1400.2585219928352476No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1390.2566754071721387No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1380.2548288215090298No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.24744247885659415No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1310.24190272186726744No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1310.24190272186726744No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.2382095505410496No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.2345163792148318No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.23266979355172288No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.23266979355172288No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.23082320788861396No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1240.22897662222550502No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1120.2068175942681981No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1060.19573808028954462No Hit
GGACTACAAGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT990.18281198064778226No Hit
GGACTACACGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT850.15695978136425748No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG770.1421870960593862No Hit
GGACTACACGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT760.1403405103962773No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA740.13664733907005946No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG730.13480075340695055No Hit
GGACTACAAGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT680.125567825091406No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG680.125567825091406No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG670.12372123942829708No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT650.12002806810207925No Hit
GGACTACTCGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT630.11633489677586144No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA610.11264172544964361No Hit
GGACTACAGGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT600.1107951397865347No Hit
GGACTACAGGGGTATCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT590.10894855412342577No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG580.10710196846031687No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG570.10525538279720797No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT570.10525538279720797No Hit
GGACTACTAGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT550.10156221147099014No Hit
GGACTACCAGGGTTTCTATCAACTTTAATGGTTCTCTTCCTCCTCCTGCT550.10156221147099014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCC108.442807E-4295.0295
ACACGGG8050.0295.06
CACGGGT8000.0295.07
GGACTAC52300.0295.01
AGGGTAT9950.0295.09
ACGGGTA3600.0295.08
CGTTAGC900.0295.0295
CAGGGTA2450.0295.08
CGGGGTA2700.0295.08
CAAGGGT7000.0295.07
CTACCCG4700.0295.04
GGGTTAT108.442807E-4295.09
ACCCGGG4700.0295.06
TACACGG8000.0295.05
ACAAGGG7000.0295.06
TACAAGG7000.0295.05
AGGGGTA2400.0294.999978
CTACAAG7050.0294.999974
GGTTAGC303.6379788E-12294.99997295
GGGGTAT7800.0293.108959
Produced by FastQC (version 0.11.5)