FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69584
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.1020349505633479No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC700.10059783858358243No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC700.10059783858358243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA1100.0295.000032
CGGGGGC550.0295.000039
TCGTCCT350.0295.000031
CATACCT350.0295.000031
CGGTCCT350.0295.000031
TCGACCT350.0295.000031
TCGCCTA1000.0295.02
GCCCCTA950.0295.02
TCGCCCT255.2568794E-10295.01
CGGGTGG108.447968E-4295.09
TTCTACG108.447968E-4295.03
AATCCTA850.0295.02
AGTCCTA850.0295.02
AATCCCT255.2568794E-10295.01
GTCGCCT157.2112125E-6295.01
AAATCCT255.2568794E-10295.01
ACGCCTA800.0295.02
GCGACCT206.143455E-8295.01
GTACAGT255.2568794E-10295.0295
GAGTCCT157.2112125E-6295.01
Produced by FastQC (version 0.11.5)