FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79374
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.15874215738151032No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.1549625822057601No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.15370272381384334No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14488371507042608No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.14110413989467585No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.1398442815027591No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.13606470632700884No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13480484793509212No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13480484793509212No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13480484793509212No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13480484793509212No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.13354498954317534No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.13354498954317534No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.13354498954317534No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.1322851311512586No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.12850555597550833No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.12850555597550833No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.12850555597550833No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1272456975835916No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1272456975835916No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.12598583919167486No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.12598583919167486No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.12472598079975811No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.12472598079975811No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.12472598079975811No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.12346612240784136No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.12346612240784136No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1222062640159246No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1222062640159246No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1222062640159246No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.12094640562400787No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.12094640562400787No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11968654723209111No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11968654723209111No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11968654723209111No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11842668884017436No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11842668884017436No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11842668884017436No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11716683044825761No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11716683044825761No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11716683044825761No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11590697205634086No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11590697205634086No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11590697205634086No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11464711366442411No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11464711366442411No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11464711366442411No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11338725527250738No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11338725527250738No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11338725527250738No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11212739688059063No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11086753848867387No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10960768009675712No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10834782170484036No Hit
CCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10834782170484036No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10834782170484036No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10834782170484036No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10708796331292364No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10708796331292364No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10708796331292364No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10708796331292364No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10582810492100689No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10456824652909014No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10456824652909014No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10456824652909014No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10456824652909014No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10330838813717338No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10204852974525663No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10204852974525663No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10204852974525663No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10204852974525663No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.10204852974525663No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10078867135333988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT450.0295.01
GCCCCTA850.0295.02
GCCCCCT206.148912E-8295.01
GTCGCCT350.0295.01
TGCCCCT206.148912E-8295.01
CGAACCT108.450209E-4295.01
ACGCCTA1300.0295.02
AGCCCTA1000.0295.02
GATCCTA1150.0295.02
GATCCCT550.0295.01
GGTCCCT108.450209E-4295.01
GCGCCTA650.0295.02
GCGCCCT350.0295.01
TAGCCCT255.275069E-10295.01
AAGCCTA900.0295.02
AGGCCTA1250.0295.02
AGTGCCT108.450209E-4295.01
TACGCCT400.0295.01
GGGACCT350.0295.01
CTCGGGA550.0295.06
Produced by FastQC (version 0.11.5)