FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91899
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1100.0295.02
CGGGTGG108.4523746E-4295.09
GTCGCCT350.0295.01
CAACGGG108.4523746E-4295.06
GGTCCTA1950.0295.02
ATAGCCT400.0295.01
ATCACGC108.4523746E-4295.0295
GGGACCT206.154187E-8295.01
CTGCCTA800.0295.02
CGGGCCT255.275069E-10295.01
GATGCCT206.154187E-8295.01
CGGTCCT550.0295.01
GTGCCTA900.0295.02
CCCTCGG303.6379788E-12294.999974
GACCCTA1050.0294.999972
GTGTCGG303.6379788E-12294.99997295
CGAGGCA157.2187395E-6294.999979
GCCTACG16100.0291.335424
CCTACGG86050.0290.71475
ACCTACG25150.0290.308174
Produced by FastQC (version 0.11.5)