FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86271
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.11939122068829618No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.11939122068829618No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.10895897810388196No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.1043224258441423No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.10084501164933754No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.10084501164933754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCT700.0295.000031
TTAGCCT350.0295.000031
GGTCCCT350.0295.000031
TATTCCT350.0295.000031
ATCCCCT350.0295.000031
CTACCTA1400.0295.000032
TACTCCT350.0295.000031
TGCTCCT350.0295.000031
AGGGCCT350.0295.000031
TGATCCT255.275069E-10295.01
TCGCCTA1000.0295.02
GCCCCTA1200.0295.02
GCCCCCT206.152004E-8295.01
AGTCCTA1500.0295.02
TTGGCCT303.6379788E-12295.01
GTCGCCT157.2172134E-6295.01
CAAACCT303.6379788E-12295.01
AGATCCT303.6379788E-12295.01
ACGCCTA650.0295.02
CCCTCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)