FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94502
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1590.16825040739878522No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1580.16719222873589976No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1570.16613405007301432No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1560.1650758714101289No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1550.16401769274724345No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1550.16401769274724345No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1540.162959514084358No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1530.16190133542147256No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1520.16084315675858712No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1510.1597849780957017No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1510.1597849780957017No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1470.1555522634441599No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1470.1555522634441599No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1460.15449408478127447No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1460.15449408478127447No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1430.15131954879261814No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1420.1502613701297327No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1410.14920319146684727No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1400.14814501280396183No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1400.14814501280396183No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1390.14708683414107637No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1390.14708683414107637No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.14602865547819094No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1370.1449704768153055No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1370.1449704768153055No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1360.14391229815242007No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.1428541194895346No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.1428541194895346No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.1428541194895346No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.1407377621637637No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.1407377621637637No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.1407377621637637No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.13862140483799285No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.13862140483799285No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.13862140483799285No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.13862140483799285No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1300.1375632261751074No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.13650504751222195No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.13650504751222195No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.13650504751222195No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.13650504751222195No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.13544686884933652No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.13544686884933652No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.13438869018645108No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.13333051152356565No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.13333051152356565No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.13333051152356565No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1250.1322723328606802No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1250.1322723328606802No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13121415419779475No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13121415419779475No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13121415419779475No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.13015597553490932No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.13015597553490932No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.13015597553490932No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.12909779687202388No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.12909779687202388No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.12909779687202388No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.12909779687202388No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.126981439546253No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.1248650822204821No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.12380690355759667No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.12380690355759667No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.12274872489471123No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.12169054623182578No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12063236756894033No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12063236756894033No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12063236756894033No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12063236756894033No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12063236756894033No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.11957418890605491No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.11851601024316946No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.11851601024316946No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.11851601024316946No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.11851601024316946No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.11851601024316946No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.11745783158028401No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.11745783158028401No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.11745783158028401No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.11639965291739857No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.11639965291739857No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.11639965291739857No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.11639965291739857No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
CTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11534147425451312No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.1142832955916277No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.1142832955916277No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.1142832955916277No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.1142832955916277No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.1142832955916277No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11322511692874225No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11322511692874225No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11322511692874225No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.11322511692874225No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.1121669382658568No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.1121669382658568No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.1121669382658568No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11110875960297137No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11005058094008592No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11005058094008592No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11005058094008592No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11005058094008592No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.10899240227720049No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.10793422361431504No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.10793422361431504No Hit
GATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.10793422361431504No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.10793422361431504No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.10793422361431504No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1068760449514296No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1068760449514296No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1068760449514296No Hit
AGCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10581786628854416No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10581786628854416No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10581786628854416No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10475968762565871No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10475968762565871No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10475968762565871No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10475968762565871No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10475968762565871No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10370150896277328No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10370150896277328No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10370150896277328No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10370150896277328No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10370150896277328No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10264333029988784No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
TGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1015851516370024No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10052697297411695No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10052697297411695No Hit
CTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10052697297411695No Hit
AGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10052697297411695No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10052697297411695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT206.155096E-8295.01
AGATCCT400.0295.01
CGAACCT450.0295.01
ACGCCTA450.0295.02
GCGACCT450.0295.01
TCCGCCT450.0295.01
TGGACCT550.0295.01
GTCTCGT108.45276E-4295.0295
CGACCTA1300.0295.02
AGGACCT500.0295.01
GCGCCTA1350.0295.02
CCTACGA108.45276E-4295.05
TAGCCCT255.275069E-10295.01
ACGGGGC255.275069E-10295.08
ACCTCGG206.155096E-8295.04
GCACCCT255.275069E-10295.01
GTTACCT400.0295.01
GTAGCCT255.275069E-10295.01
TGACCTA1600.0295.02
TGCGCCT400.0295.01
Produced by FastQC (version 0.11.5)