FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81963
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCT255.275069E-10295.01
ATTACCT350.0295.01
CGCGCCT255.275069E-10295.01
GCGCCCT255.275069E-10295.01
GACCCTA1250.0295.02
ACCCTCG108.450715E-4295.03
CCGTCCT500.0295.01
TACGCCT350.0295.01
CTGCCTA650.0295.02
ATCCCCT350.0295.01
AACGCCT450.0295.01
AGCGCCT255.275069E-10295.01
GAGCCTA1150.0295.02
CTCGCCT108.450715E-4295.01
GACGCCT255.275069E-10295.01
GGCGCCT350.0295.01
CGCACCT255.275069E-10295.01
CGTCCTA1350.0295.02
TTCGCCT350.0295.01
GGTTCCT350.0295.01
Produced by FastQC (version 0.11.5)