FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67273
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.15905340924293548No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.15162100694186376No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.14864804602143505No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.14864804602143505No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.14864804602143505No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.1471615655612207No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.14567508510100635No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.14567508510100635No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.14270212418057765No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1412156437203633No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.13972916326014895No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13675620233972025No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13675620233972025No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.13675620233972025No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1352697218795059No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1352697218795059No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1352697218795059No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1352697218795059No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.1352697218795059No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.13378324141929154No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.1322967609590772No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.13081028049886284No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.13081028049886284No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.13081028049886284No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.12783731957843414No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.12783731957843414No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.12783731957843414No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.1263508391182198No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.1263508391182198No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.12486435865800545No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.12486435865800545No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.12337787819779109No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.12337787819779109No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.12337787819779109No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.12189139773757673No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.12189139773757673No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.12189139773757673No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1204049172773624No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1204049172773624No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.11891843681714803No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.11594547589671934No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.11594547589671934No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.11594547589671934No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.11594547589671934No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11445899543650498No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11445899543650498No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11445899543650498No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.11445899543650498No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.11297251497629063No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.11148603451607629No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.10999955405586194No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10851307359564757No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.10851307359564757No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10702659313543324No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.10702659313543324No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10554011267521889No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10405363221500452No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10405363221500452No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC700.10405363221500452No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10256715175479018No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10256715175479018No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10256715175479018No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.10256715175479018No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10108067129457583No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10108067129457583No Hit
AGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10108067129457583No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10108067129457583No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC680.10108067129457583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA1400.0295.000032
AACGCCT350.0295.000031
CTACCTA700.0295.000032
GGGCCTA700.0295.000032
AGCCTAC3550.0295.000033
GACGCCT350.0295.000031
CGATCCT350.0295.000031
TGTCCTA1400.0295.000032
GTTGATT206.1419996E-8295.0295
GCCCCTA400.0295.02
AATCCTA1050.0295.02
AGTCCTA900.0295.02
CCCCTCG108.447348E-4295.03
TGCCCCT108.447348E-4295.01
AGATCCT400.0295.01
CGGCCTA950.0295.02
AGCCCTA950.0295.02
TAGACCT206.1419996E-8295.01
GGTCCTA1250.0295.02
GGTCCGG255.2568794E-10295.0295
Produced by FastQC (version 0.11.5)