FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90792
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1690.18613974799541808No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1660.18283549211384262No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1630.17953123623226716No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1600.1762269803506917No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1600.1762269803506917No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1570.17292272446911622No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1520.1674156313331571No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1510.16631421270596528No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1510.16631421270596528No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1500.16521279407877346No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1490.16411137545158164No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1420.15640144506123888No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1410.15530002643404706No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1380.1519957705524716No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1380.1519957705524716No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1370.15089435192527978No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1350.1486915146708961No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1350.1486915146708961No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1350.1486915146708961No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1350.1486915146708961No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1340.1475900960437043No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1340.1475900960437043No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1340.1475900960437043No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1320.14538725878932066No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.14428584016212884No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1300.143184421534937No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1300.143184421534937No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.14208300290774517No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.14208300290774517No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.14208300290774517No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1280.14098158428055335No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.13988016565336153No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.13988016565336153No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.13988016565336153No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.1387787470261697No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.1387787470261697No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.1387787470261697No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.1387787470261697No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1250.1376773283989779No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.13657590977178605No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.13657590977178605No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.13657590977178605No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.13547449114459423No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.13547449114459423No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.1343730725174024No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.1343730725174024No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.1343730725174024No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.1332716538902106No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1200.13217023526301877No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1200.13217023526301877No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.13106881663582692No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.13106881663582692No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1180.1299673980086351No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1180.1299673980086351No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1170.12886597938144329No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1160.12776456075425147No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1160.12776456075425147No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1150.12666314212705965No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1150.12666314212705965No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1140.12556172349986783No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1130.124460304872676No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1130.124460304872676No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1130.124460304872676No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1130.124460304872676No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1120.12335888624548418No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1110.12225746761829236No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1110.12225746761829236No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1100.12115604899110054No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1100.12115604899110054No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1090.12005463036390872No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1090.12005463036390872No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1090.12005463036390872No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1090.12005463036390872No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1080.11895321173671689No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.11785179310952507No Hit
GTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.11785179310952507No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.11785179310952507No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1070.11785179310952507No Hit
GCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1060.11675037448233325No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1050.11564895585514143No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1050.11564895585514143No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1050.11564895585514143No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1050.11564895585514143No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1030.11344611860075779No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.11234469997356594No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.11234469997356594No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.11234469997356594No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1020.11234469997356594No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1010.11124328134637412No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1010.11124328134637412No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1010.11124328134637412No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1000.1101418627191823No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.10904044409199048No Hit
GATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.10904044409199048No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.10904044409199048No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC990.10904044409199048No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC980.10793902546479867No Hit
ACTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC980.10793902546479867No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC980.10793902546479867No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.10683760683760685No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.10683760683760685No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.10683760683760685No Hit
AGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.10683760683760685No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC970.10683760683760685No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC960.10573618821041501No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC960.10573618821041501No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC960.10573618821041501No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC960.10573618821041501No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.1046347695832232No Hit
CCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.1046347695832232No Hit
AGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.1046347695832232No Hit
CAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC950.1046347695832232No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC940.10353335095603138No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC940.10353335095603138No Hit
CGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC940.10353335095603138No Hit
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC940.10353335095603138No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
GCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC930.10243193232883956No Hit
TAGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
GGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
GGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
ATTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC920.10133051370164774No Hit
GCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit
ACCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit
CCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit
GAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC910.10022909507445589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCT550.0295.000031
CGGCCTA1100.0295.000032
GGTCCTA1250.0295.000032
GTCCCCT550.0295.000031
TGATCCT450.0295.01
GCCCCCT206.153823E-8295.01
GTCGCCT350.0295.01
CCCCTAG108.452209E-4295.03
TGCCCCT255.275069E-10295.01
GCGACCT255.275069E-10295.01
CCCTCCT450.0295.01
CAGCCTA1150.0295.02
CTATCCT500.0295.01
CGTGCCT400.0295.01
AGCCCTA800.0295.02
CCGGCCT350.0295.01
GATCCTA1350.0295.02
TTGTCCT350.0295.01
GGTCCCT450.0295.01
CACGCCT500.0295.01
Produced by FastQC (version 0.11.5)