FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59968
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC700.11672892209178229No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC690.11506136606189968No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC680.11339381003201707No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC680.11339381003201707No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC670.11172625400213447No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC630.10505602988260405No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC620.10338847385272146No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC620.10338847385272146No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC620.10338847385272146No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.10172091782283885No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.10172091782283885No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.10172091782283885No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC610.10172091782283885No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.10005336179295624No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.10005336179295624No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.10005336179295624No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC600.10005336179295624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA700.0295.000032
AGAACCT350.0295.000031
CTGCCTA350.0295.000032
CCCCCTA700.0295.000032
GGGCCTA700.0295.000032
CGGACCT350.0295.000031
GCCCCTA750.0295.02
AGTCCTA1000.0295.02
TTGGCCT255.2568794E-10295.01
GTCGCCT206.136361E-8295.01
GTACCCT108.445062E-4295.01
CCCCTAC3250.0295.03
AGATCCT206.136361E-8295.01
ACGCCTA650.0295.02
CGGCCTA400.0295.02
AGCCCTA500.0295.02
GGTCCTA650.0295.02
CGCGCCT206.136361E-8295.01
GGATCCT108.445062E-4295.01
GCGCCTC108.445062E-4295.02
Produced by FastQC (version 0.11.5)