FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125529
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1500.0295.02
GCGCCCT350.0295.01
TACGGGC108.4560603E-4295.07
TACGCCT600.0295.01
CTACCGG108.4560603E-4295.06
CGAGCCT206.1631E-8295.01
GACGCCT157.225035E-6295.01
CGATCCT850.0295.01
GTTGCCT157.225035E-6295.01
GCCTACG22850.0290.48144
TCCTACG33300.0290.127664
CCTACGG117200.0289.08495
GTCCTAC8800.0288.295443
CTACGGG115250.0288.216926
CGCCTAC6400.0288.085943
ACCTACG33200.0286.114444
TACGGGA114650.0285.994357
ACGGGAG114950.0285.119638
CGGGAGG113100.0284.30599
CCCTACG28850.0281.195834
Produced by FastQC (version 0.11.5)