FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125529
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA32862.6177218013367427No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA26122.0807940794557434No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA19221.531120298895076No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15811.2594699232846593No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14531.157501453847318No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG13811.1001441897888138No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT11400.9081566809263197No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10140.8077814688239371No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG10080.8030016968190617No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9930.7910522668068733No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA9030.7193556867337427No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT8860.7058129993865959No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG8150.6492523639955707No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7330.5839288132622741No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7080.5640130965752933No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6770.5393176078834373No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6700.5337412072110826No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6400.5098423471867058No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6220.4955030311720798No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5510.4389423957810546No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA5430.4325693664412208No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4920.3919413043997801No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4920.3919413043997801No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4830.3847716463924671No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4720.37600873105019555No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4650.37043233037784096No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4310.34334695568354723No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4230.3369739263437134No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4100.32661775366648343No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3870.3082952943144612No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3850.3067020369795027No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3850.3067020369795027No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3850.3067020369795027No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3800.30271889364210663No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC3710.2955492356347936No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3680.2931593496323559No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3670.29236272096487664No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3550.2828031769551259No Hit
GGACTACAAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3510.279616662285209No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3510.279616662285209No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3420.2724470042778959No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3230.25731105959579065No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3230.25731105959579065No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3180.25332791625839446No Hit
GGACTACTAGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3070.24456500091612296No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3010.2397852289112476No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2940.234208828238893No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2930.23341219957141376No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2920.23261557090393453No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2890.23022568490149686No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2830.22544591289662147No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2810.22385265556166306No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2690.21429311155191233No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2690.21429311155191233No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2680.2134964828844331No Hit
GGACTACAAGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG2660.21190322554947463No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2650.2111065968819954No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2590.20632682487712004No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2590.20632682487712004No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2540.20234368153972387No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2490.19836053820232774No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC2480.1975639095348485No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2430.1935807661974524No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2430.1935807661974524No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2320.18481785085518088No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2310.18402122218770164No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.18402122218770164No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2300.1832245935202224No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA2280.18163133618526395No Hit
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC2240.17844482151534705RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2210.17605493551290935No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2190.1744616781779509No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG2140.17047853484055478No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2140.17047853484055478No Hit
GGACTACTAGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG2110.16808864883811708No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2030.16171561949828325No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2030.16171561949828325No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2000.15932573349584558No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2000.15932573349584558No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1970.1569358474934079No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1950.15534259015844942No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1910.15215607548853255No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1900.15135944682105332No Hit
GGACTACACGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1880.14976618948609485No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1850.14737630348365716No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1830.14578304614869872No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1800.14339316014626102No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1760.14020664547634412No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1740.13861338814138566No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1710.13622350213894796No Hit
GGACTACTGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1700.13542687347146873No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1680.1338336161365103No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1670.13303698746903106No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1660.13224035880155185No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1660.13224035880155185No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1640.1306471014665934No Hit
GGACTACACGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1580.12586732946171802No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1550.12347744345928033No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1520.12108755745684265No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1520.12108755745684265No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1520.12108755745684265No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.12029092878936341No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1510.12029092878936341No Hit
GGACTACAAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1500.11949430012188418No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.11949430012188418No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1490.11869767145440495No Hit
GGACTACAAGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA1490.11869767145440495No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1480.11790104278692573No Hit
GGACTACAAGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG1480.11790104278692573No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1480.11790104278692573No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1460.11630778545196727No Hit
GGACTACTCGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1450.11551115678448803No Hit
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC1430.11391789944952958RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1430.11391789944952958No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.10754487010969577No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1340.10674824144221655No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1340.10674824144221655No Hit
GGACTACAAGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT1330.10595161277473732No Hit
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC1310.10435835543977885RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1300.10356172677229963No Hit
GGACTACAAGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1300.10356172677229963No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1290.1027650981048204No Hit
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC1280.10196846943734117RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1270.10117184076986194No Hit
GGACTACTAGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA1270.10117184076986194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1550.0295.000038
CGGGTCT108.4560603E-4295.09
ACGGCTT108.4560603E-4295.08
GGGGTAT1650.0295.09
GGACTAC118350.0294.376861
TACTCGG14050.0292.900365
GGGGTTT19200.0292.69539
ACACGGG14750.0292.06
TCGGGTT9600.0291.92718
TACAAGG25600.0291.542975
ACAAGGG22800.0291.118446
CACGGGT14800.0291.013527
CAAGGGT22850.0289.83597
CTACTCG14200.0289.806374
GACTACC22400.0289.732152
TACACGG16750.0289.716435
ACGGGTT13200.0289.41298
CTACAAG25900.0289.305024
CTACTAG23750.0288.789464
CTACCCG8850.0288.33334
Produced by FastQC (version 0.11.5)