FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69046
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC880.127451264374475No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC840.12165802508472612No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC830.1202097152622889No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11731309561741447No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.11731309561741447No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.11731309561741447No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.11586478579497728No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11296816615010283No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC770.11151985632766562No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC770.11151985632766562No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1100715465052284No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.1100715465052284No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC750.1086232366827912No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.10717492686035397No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10717492686035397No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC740.10717492686035397No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10572661703791675No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10427830721547954No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10427830721547954No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10427830721547954No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10427830721547954No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC720.10427830721547954No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10427830721547954No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.10282999739304231No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10282999739304231No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC710.10282999739304231No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC700.10138168757060512No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10138168757060512No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC700.10138168757060512No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.10138168757060512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT206.121081E-8295.211761
ATCCCCT303.6379788E-12295.211761
ACAGCCT157.190318E-6295.211761
AGCCTAC3400.0295.211763
TTGTCCT400.0295.211761
AATCCTA950.0295.211762
CCTACCT157.190318E-6295.211761
GGCACCT400.0295.211761
ATCTCCT400.0295.211761
AGCTCCT255.2386895E-10295.211761
CGCCCTA850.0295.211762
TTCGCCT303.6379788E-12295.211761
TGTCCTA1000.0295.211762
TTACCCT303.6379788E-12295.211761
GGACCTA650.0295.211762
AGGACCT255.2386895E-10295.211761
GTTCCTA950.0295.211762
GTCGCCT255.2386895E-10295.211761
GGTCCTA950.0295.211762
GGCGCCT108.429675E-4295.211761
Produced by FastQC (version 0.11.5)