FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74257
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.146183E-8295.01
TCGCCTA950.0295.02
GCCCCTA1000.0295.02
TGCCCCT350.0295.01
ACCGCCT206.146183E-8295.01
GTCCTAG108.4491173E-4295.03
TGGCGTT108.4491173E-4295.0295
GATCCTA600.0295.02
CGCGCCT157.2131734E-6295.01
GCGCCTA600.0295.02
TACGGCG108.4491173E-4295.07
CGTTCCT157.2131734E-6295.01
TACGCCT255.2568794E-10295.01
TGCGCCT206.146183E-8295.01
CTACGAG108.4491173E-4295.06
CGGGCGG108.4491173E-4295.08
CGGGCCT303.6379788E-12295.01
CGTACCT206.146183E-8295.01
CGAGCCT255.2568794E-10295.01
TTGCCTA1100.0295.02
Produced by FastQC (version 0.11.5)