FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74257
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT11391.5338621274761977No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT9231.242980459754636No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7050.9494054432578747No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6460.8699516543894852No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6440.8672583056142855No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6070.8174313532730921No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT6020.8106979813350931No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5830.7851111679706964No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5800.7810711448078969No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC5400.727204169303904No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5040.6787238913503104No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4970.6692971706371117No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA4970.6692971706371117No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4800.6464037060479146No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4730.6369769853347159No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC4680.6302436133967168No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4360.5871500329935225No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3850.5184696392259315No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3850.5184696392259315No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3840.5171229648383318No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3770.5076962441251329No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3720.5009628721871339No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3670.49422950024913476No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC3640.49018947708633526No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3630.4888428026987354No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3480.46864268688473815No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3470.4672960124971383No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3460.46594933810953854No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3350.4511359198459405No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC3240.4363225015823424No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3230.4349758271947426No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3210.4322824784195429No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3050.41073568821794576No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3010.40534899066754654No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2980.40130896750474704No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2960.39861561872954737No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2960.39861561872954737No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2940.3959222699543477No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2880.3878422236287488No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2750.3703354565899511No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2700.363602084651952No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2680.3609087358767524No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2660.3582153871015527No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2640.3555220383263531No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2610.35148201516355365No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2600.3501353407759538No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2600.3501353407759538No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2590.348788666388354No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2530.34070862006275504No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2530.34070862006275504No Hit
GGACTACAAGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG2490.33532192251235576No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2480.33397524812475593No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2460.33128189934955626No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2430.3272418761867568No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2420.325895201799157No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2350.3164684810859582No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2340.3151218066983584No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2330.31377513231075854No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2210.2976150396595607No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2200.29626836527196093No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2200.29626836527196093No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2170.29222834210916143No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2130.28684164455876215No Hit
GGACTACACGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG2100.28280162139596265No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2090.2814549470083629No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2060.2774149238455634No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2060.2774149238455634No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2030.27337490068276393No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2020.2720282262951641No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1960.26394817996956516No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1960.26394817996956516No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1940.2612548311943655No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1900.2558681336439662No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1890.2545214592563664No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1870.25182811048116677No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1820.24509473854316763No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1790.24105471538036818No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1790.24105471538036818No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1770.23836136660516852No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1720.23162799466716943No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1720.23162799466716943No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1630.21950792517877105No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1620.2181612507911712No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1560.21008120446557227No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1540.20738785569037263No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1490.20065448375237352No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1480.19930780936477369No Hit
GGACTACAAGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT1470.19796113497717385No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1470.19796113497717385No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1440.1939211118143744No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1420.19122776303917477No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1420.19122776303917477No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1400.1885344142639751No Hit
GGACTACAAGGGTTTCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1400.1885344142639751No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1400.1885344142639751No Hit
GGACTACCAGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1400.1885344142639751No Hit
GGACTACAGGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1390.1871877398763753No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1360.18314771671357583No Hit
GGACTACCCGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1340.1804543679383762No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1340.1804543679383762No Hit
GGACTACACGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT1340.1804543679383762No Hit
GGACTACAAGGGTATCTAATGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1320.17776101916317655No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1320.17776101916317655No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1310.17641434477557671No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1280.17237432161277724No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1260.1696809728375776No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1250.16833429844997777No Hit
GGACTACTCGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1250.16833429844997777No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1240.16698762406237797No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1240.16698762406237797No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1230.16564094967477813No Hit
GGACTACTAGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1210.1629476008995785No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1200.16160092651197866No Hit
GGACTACCAGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT1200.16160092651197866No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1180.15890757773677902No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1170.1575609033491792No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1170.1575609033491792No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1150.15486755457397955No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1140.15352088018637972No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1130.1521742057987799No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1120.1508275314111801No Hit
GGACTACAGGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT1110.14948085702358027No Hit
GGACTACACGGGTATCTAATGAGGCACCTCCCGCTTTGCTTTCTTTCCCG1050.14140081069798133No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1010.13601411314758205No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1010.13601411314758205No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTCGCATGTCTCCTGATCTTA990.13332076437238238No Hit
GGACTACACGGGTTTCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG990.13332076437238238No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC990.13332076437238238No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC970.13062741559718274No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG950.1279340668219831No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTCGCATGTCTCCTGATCTTA940.12658739243438327No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA940.12658739243438327No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC930.12524071804678347No Hit
GGACTACAAGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG930.12524071804678347No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC930.12524071804678347No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC930.12524071804678347No Hit
GGACTACACGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG920.12389404365918365No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC910.12254736927158381No Hit
GGACTACTAGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT900.12120069488398401No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA880.11850734610878436No Hit
GGACTACAAGGGTTTCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT870.11716067172118454No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA860.11581399733358472No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA850.11446732294598488No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC850.11446732294598488No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA850.11446732294598488No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC840.11312064855838508No Hit
GGACTACCGGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG830.11177397417078525No Hit
GGACTACTCGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT820.11042729978318541No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG820.11042729978318541No Hit
GGACTACAGGGGTTTCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG810.1090806253955856No Hit
GGACTACAAGGGTATCTAATAGCTGACGCTTAGCATGTCTCCTGATCTTA810.1090806253955856No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA810.1090806253955856No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA800.10773395100798577No Hit
GGACTACCAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC790.10638727662038594No Hit
GGACTACTGGGGTATCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG790.10638727662038594No Hit
GGACTACACGGGTTTCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT780.10504060223278613No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTCGCATGTCTCCTGATCTTA770.10369392784518632No Hit
GGACTACCCGGGTATCTAATTCCCTGTGTGTCCTTGGCATGGCCGTGATT760.1023472534575865No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA750.10100057906998668No Hit
GGACTACTAGGGTTTCTAAAGAGGCACCTCCCGCTTTGCTTTCTTTCCCG750.10100057906998668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA4700.0295.08
TGTTCAC108.4491173E-4295.0295
ACTACCG5950.0295.03
GGACTAC70650.0294.582431
CTACTCG6500.0292.730774
TACTCGG6500.0292.730775
CTACCGG5850.0292.478644
CGGGGTA4100.0291.402448
GACTACC20850.0290.75542
GGGGTAT12700.0290.35439
CGGGTAT15850.0290.347029
ACCGGGG5900.0290.000036
TACCGGG5900.0290.000035
TCGGGTT2300.0288.586948
CAAGGGT12450.0287.891577
CTACCCG7200.0286.805574
ACCCGGG7150.0286.748266
ACGGGTA7000.0286.57148
GACTACA31400.0286.54462
AGGGTAT18600.0286.27699
Produced by FastQC (version 0.11.5)