FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68132
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC720.10567721481829391No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10420947572359537No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTC690.10127399753419833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCT108.447581E-4295.01
ATCCCCT450.0295.01
ATCCTCG108.447581E-4295.03
AATCCTA1550.0295.02
GTTGAGA108.447581E-4295.0295
ACGCCTA750.0295.02
TGCACCT206.142545E-8295.01
GGGTCCT206.142545E-8295.01
GTCACCT400.0295.01
CCTACCT400.0295.01
ATCTCCT450.0295.01
TGACCTA1100.0295.02
TCGACCT400.0295.01
GAAACCT206.142545E-8295.01
TGCGCCT255.2568794E-10295.01
TTACCCT450.0295.01
CGCCCCT206.142545E-8295.01
GGACCTA900.0295.02
CGCCTCG108.447581E-4295.03
AATTCCT400.0295.01
Produced by FastQC (version 0.11.5)