FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67135
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.02
CGGGTGG157.210081E-6295.09
CCGGCCT255.2568794E-10295.01
GTCCTCG108.4473053E-4295.03
GAGTACT108.4473053E-4295.0295
GGTCCTA900.0295.02
CGCGCCT157.210081E-6295.01
CCTACGT108.4473053E-4295.05
GCGCCTA800.0295.02
ACGGGTG157.210081E-6295.08
CGTTCCT206.141818E-8295.01
GTTACCT157.210081E-6295.01
TTCCCTA800.0295.02
ACCTATG108.4473053E-4295.04
TACGCCT108.4473053E-4295.01
TGCGCCT303.6379788E-12295.01
ACAGCCT206.141818E-8295.01
CTCGCCT206.141818E-8295.01
CGAGCCT350.0295.01
ACGTCCT550.0295.01
Produced by FastQC (version 0.11.5)