FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50496
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC530.10495880861850443No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC520.10297845373891001No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC510.10099809885931559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA550.0295.000032
GCGCCTA550.0295.000032
CGCCTAC2650.0295.000033
CGTCCTA550.0295.000032
CGGGTGC108.4410975E-4295.09
AGTCCTA700.0295.02
GTACCTC108.4410975E-4295.02
CAAACCT206.126902E-8295.01
TCACCCT108.4410975E-4295.01
CTATCCT350.0295.01
TTAGCCT108.4410975E-4295.01
AGCCCTA400.0295.02
TAGACCT255.2386895E-10295.01
GACACCT255.2386895E-10295.01
GGGTCCT255.2386895E-10295.01
GATCCTA650.0295.02
CTCCCTA900.0295.02
GATCCCT108.4410975E-4295.01
GGTCCCT255.2386895E-10295.01
CGCGCCT206.126902E-8295.01
Produced by FastQC (version 0.11.5)