FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108277
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.10159128900874609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCT350.0295.000031
GCGCCCT350.0295.000031
AGTGCCT350.0295.000031
TATACCT350.0295.000031
ACGACCT350.0295.000031
CGGACCT350.0295.000031
TTGGCCT255.275069E-10295.01
CGAACCT500.0295.01
CAACGGG157.2222956E-6295.06
CTTCCCT255.275069E-10295.01
CGCGCCT255.275069E-10295.01
CGTTCCT500.0295.01
TAGCCCT450.0295.01
GCACCTA1500.0295.02
CTGCGGG157.2222956E-6295.06
CGCCTAG157.2222956E-6295.03
ATTCCCT550.0295.01
AAGCCTA1250.0295.02
TACGCCT750.0295.01
TGCGCCT157.2222956E-6295.01
Produced by FastQC (version 0.11.5)