FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113887
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG206.160553E-8295.03
AGATCCT450.0295.01
GAGTCCT450.0295.01
GTCCTCG108.455032E-4295.03
CCTACGC206.160553E-8295.05
GCGCCTA1600.0295.02
GCGCCCT500.0295.01
CGTTCCT255.275069E-10295.01
TTCCCTA1550.0295.02
GTAGCCT255.275069E-10295.01
TACGCCT650.0295.01
TCGTCCT500.0295.01
GTTCCCT400.0295.01
GAGCCTA1500.0295.02
CTACCGG157.223276E-6295.06
CTCGCCT450.0295.01
GCGGCCT108.455032E-4295.01
GGCGCCT255.275069E-10295.01
ACGACCT500.0295.01
GTTGCCT157.223276E-6295.01
Produced by FastQC (version 0.11.5)