FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62398
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA700.0295.000032
TAAGCCT350.0295.000031
GTCGCCT108.445872E-4295.01
ACGCCTA750.0295.02
GATCCTA850.0295.02
CGCGCCT255.2568794E-10295.01
CCTACGT157.2076327E-6295.05
GCGCCTA750.0295.02
CCTACGA108.445872E-4295.05
GCGCCCT206.1383616E-8295.01
TAGCCTA950.0295.02
TAGCCCT303.6379788E-12295.01
GACCCCT303.6379788E-12295.01
GCACCCT303.6379788E-12295.01
CGGGGCA157.2076327E-6295.09
AAGCCTA850.0295.02
GTAGCCT206.1383616E-8295.01
TACGCCT157.2076327E-6295.01
TCTACGG108.445872E-4295.04
TATTCCT157.2076327E-6295.01
Produced by FastQC (version 0.11.5)