FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67749
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT157.210365E-6295.01
TCGCCTA1100.0295.02
GCCCCTA950.0295.02
TCGCCCT255.2568794E-10295.01
AGTCCTA750.0295.02
GTCGCCT303.6379788E-12295.01
TGCCCCT255.2568794E-10295.01
CGAACCT350.0295.01
ACGCCTA450.0295.02
CCCTCCT303.6379788E-12295.01
TCCGCCT255.2568794E-10295.01
GACACCT157.210365E-6295.01
GGGTCCT157.210365E-6295.01
ACCGCCT303.6379788E-12295.01
GATCCTA1050.0295.02
GGTCCTA1100.0295.02
TGGCGAT255.2568794E-10295.0295
TTGTCCT255.2568794E-10295.01
CAACCTA1100.0295.02
TCCCTAC2850.0295.03
Produced by FastQC (version 0.11.5)