FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67749
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8741.2900559417851187No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8641.2752955763184697No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6760.9978007055454694No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6630.9786122304388257No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6550.9668039380655065No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6470.9549956456921873No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6430.9490914995055277No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6390.9431873533188682No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6330.9343311340388788No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6300.9299030243988841No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6030.890050037638932No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5980.8826698549056075No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5770.8516730874256447No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5590.8251044295856765No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5540.817724246852352No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5310.7837754062790594No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5050.7453984560657722No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4930.7276860175057934No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4860.7173537616791392No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4700.6937371769325008No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4670.6893090672925062No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4670.6893090672925062No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4630.6834049211058466No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4560.6730726652791923No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4540.6701205921858625No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4530.6686445556391976No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4530.6686445556391976No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4500.6642164459992029No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4410.6509321170792188No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4350.6420758977992295No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4340.6405998612525646No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4310.6361717516125699No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4310.6361717516125699No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4300.634695715065905No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4300.634695715065905No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4280.6317436419725753No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4260.6287915688792455No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4160.6140312034125965No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4140.6110791303192668No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4100.6051749841326071No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4040.5963187648526177No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3970.5859865090259635No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3950.5830344359326337No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3940.5815583993859688No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3830.5653219973726549No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3730.550561631906006No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3710.5476095588126761No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3700.5461335222660113No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA3670.5417054126260167No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3610.5328491933460272No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3590.5298971202526975No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3580.5284210837060326No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3570.5269450471593676No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3570.5269450471593676No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3540.5225169375193729No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3510.5180888278793783No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3440.5077565720527241No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3430.5062805355060591No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3400.5018524258660644No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3360.49594827967940486No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3350.49447224313273996No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3340.492996206586075No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3300.4870920603994155No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3280.4841399873060857No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA3270.48266395075942076No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3250.479711877666091No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3230.47675980457276124No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3170.4679035852927719No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3050.45019114673279303No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3010.4442870005461335No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3010.4442870005461335No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3000.4428109639994686No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3000.4428109639994686No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2990.4413349274528037No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2960.43690681781280905No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2890.4265745619861548No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2870.423622488892825No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2860.42214645234616005No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2860.42214645234616005No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2850.42067041579949516No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2840.41919437925283026No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2800.41329023306617074No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2760.40738608687951117No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2760.40738608687951117No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2750.4059100503328463No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2750.4059100503328463No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.4044340137861814No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2710.40000590414618664No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2670.39410175795952707No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2590.3822934655862079No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2570.3793413924928781No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2570.3793413924928781No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2560.37786535594621323No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2520.37196120975955366No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2500.36900913666622387No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2460.3631049904795643No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2440.36015291738623445No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2400.35424877119957493No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2380.3512966981062451No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2360.34834462501291535No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2340.3453925519195855No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2330.3439165153729207No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2180.3217759671729472No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2170.3202999306262823No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2140.31587182098628763No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2130.31439578443962274No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2130.31439578443962274No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2130.31439578443962274No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2120.3129197478929578No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2100.30996767479962806No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2090.3084916382529631No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2060.3040635286129684No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2050.30258749206630353No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2030.29963541897297374No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2020.29815938242630885No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1980.2922552362396493No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1870.27601883422633544No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1850.27306676113300565No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1840.27159072458634076No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1780.2627345053063514No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1720.25387828602636203No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1710.2524022494796971No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1670.2464981032930375No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1660.24502206674637267No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1650.24354603019970775No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1640.24206999365304285No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1550.2287856647330588No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1530.225833591639729No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1530.225833591639729No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1490.2199294454530694No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1490.2199294454530694No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1400.20664511653308537No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1390.20516907998642045No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1390.20516907998642045No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1300.1918847510664364No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1160.17122023941312786No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1110.1638400566798034No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1060.1564598739464789No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1060.1564598739464789No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1030.1520317643064842No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA1020.15055572775981935No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA960.14169950847982996No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA960.14169950847982996No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA920.13579536229317038No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA910.13431932574650549No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG850.1254631064665161No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA820.12103499682652143No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA780.11513085063986184No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA750.11070274099986716No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA750.11070274099986716No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG740.10922670445320226No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT680.10037048517321288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCG6850.0295.04
TACAGGG7150.0295.05
GACTACA27250.0295.02
ATTTGCC108.447473E-4294.99997295
GGACTAC63900.0294.769171
CAGGGGT7200.0292.95147
TACAAGG11100.0292.342355
CAAGGGT11050.0292.330327
GGGGTAT9800.0291.98989
GACTACC19050.0291.90292
ACACGGG9300.0291.827946
TACACGG9300.0291.827945
TACTGGG4500.0291.722235
ACTGGGG4500.0291.722236
AGGGGTA4050.0291.358038
CTACAAG11150.0291.031374
ACAAGGG11100.0291.013526
ACAGGGG7250.0290.931036
CTACCAG7200.0290.902774
ACTACCA7150.0290.87413
Produced by FastQC (version 0.11.5)