FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96855
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.10118217954674515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1150.0294.99642
AATCCCT255.275069E-10294.99641
TCACCCT650.0294.99641
AGCCCTA1250.0294.99642
GGTCCTA950.0294.99642
AAGACCT600.0294.99641
GCGCCCT255.275069E-10294.99641
GCCGCCT157.220304E-6294.99641
AAGCCTA1150.0294.99642
ATCCCTA1300.0294.99642
GCCCTAC4800.0294.99643
ACTACCT305.456968E-12294.99641
GTTCCCT305.456968E-12294.99641
GGGCCCT157.220304E-6294.99641
CCGACCT305.456968E-12294.99641
TTGCCTA1150.0294.99642
AGCTCCT255.275069E-10294.99641
TTACCCT157.220304E-6294.99641
AGGGCCT305.456968E-12294.99641
CCGCCCT255.275069E-10294.99641
Produced by FastQC (version 0.11.5)