FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96855
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA35523.667337772959579No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA27652.8547829229260238No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA24472.5264570750090343No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA22052.2765990398017655No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA21012.169222032935832No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA18791.9400134221258585No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17261.782045325486552No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16341.6870579732589954No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA14611.5084404522223944No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14271.473336430746993No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA14251.4712714883072635No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13101.3525372980228176No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA12671.3081410355686334No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA12181.257549945795261No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA11041.13984822673068No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10351.0686077125600124No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10091.041763460843529No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA9871.0190490940065045No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9801.0118217954674513No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9540.9849775437509679No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9080.9374838676371896No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA9070.9364513964173248No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA8940.9230292705590831No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7510.7753858861184244No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7410.765061173919777No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7260.7495741056218058No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA7210.744411749522482No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7150.7382169222032936No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7120.7351195085436993No Hit
GGACTACTCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA6750.6969180734087037No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6740.695885602188839No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6530.6742037065716794No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6530.6742037065716794No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6340.6545867533942491No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6220.6421970987558723No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6000.6194827319188477No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5780.5967683650818233No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5360.5534045738475041No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5310.5482422177481803No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5080.5244953796912911No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5070.5234629084714264No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4990.5152031387125084No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4960.5121057250529142No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4920.5079758401734552No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4640.47906664601724225No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4600.47493676113778327No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4540.4687419338185948No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4410.4553198079603531No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4320.4460275669815704No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4260.43983273966238196No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4250.4388002684425171No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4220.435702854782923No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4170.43054049868359917No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3970.4098910742863043No Hit
GGACTACTGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA3930.4057611894068453No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3870.3995663620876568No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3710.38304682256982087No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3670.3789169376903619No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3650.3768519952506324No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3610.37272211037117337No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3540.3654948118321202No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3440.3551700996334727No Hit
GGACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA3410.3520726859738785No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3390.350007743534149No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3130.32316349181766557No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3120.32213102059780085No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3000.30974136595942386No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2990.30870889473955915No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2970.30664395229982966No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2910.3004491249806412No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2860.29528676888131744No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2730.28186464302307573No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2730.28186464302307573No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2660.27463734448402255No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2610.2694749883846988No Hit
GGACTACCGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2600.268442517164834No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2600.268442517164834No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2520.26018274740591607No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2450.25295544886686283No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2380.24572815032780962No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2340.2415982654483506No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2320.23953332300862112No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2240.23127355324970314No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2210.22817613959010893No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2120.2188838986113262No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2040.21062412885240822No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1940.20029941665376078No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1890.19513706055443705No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1810.18687729079551907No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1810.18687729079551907No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1770.1827474059160601No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.17964999225646586No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1640.16932528005781838No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1630.16829280883795364No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1630.16829280883795364No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1610.16622786639822415No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1580.1631304527386299No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1580.1631304527386299No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1580.1631304527386299No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1560.16106551029890043No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1510.15590315419957668No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1470.1517732693201177No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1440.14867585566052346No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1430.14764338444065872No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1400.1445459707810645No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.142481028341335No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1360.14041608590160548No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1310.13525372980228176No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1270.13112384492282278No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1200.12389654638376955No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.12286407516390481No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1190.12286407516390481No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1170.1207991327241753No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1170.1207991327241753No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1160.11976666150431056No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1150.11873419028444582No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1130.11666924784471634No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1110.11460430540498684No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.11253936296525735No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.1094419493056631No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1060.1094419493056631No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1050.10840947808579836No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1050.10840947808579836No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.10737700686593361No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.10737700686593361No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1030.10634453564606888No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1030.10634453564606888No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.10531206442620411No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1000.10324712198647462No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA980.10118217954674515No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)