FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81940
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.11471808640468636No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.11471808640468636No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11227727605565047No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11105687088113253No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.10861606053209666No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10739565535757872No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.10495484500854284No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.1037344398340249No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.1037344398340249No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.1037344398340249No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10251403465950697No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10251403465950697No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10007322431047107No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10007322431047107No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10007322431047107No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10007322431047107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAC3500.0294.995763
ACGCGAG108.451072E-4294.995738
ATCCTCG108.451072E-4294.995733
TTGGCCT500.0294.995731
GTCGCCT206.150549E-8294.995731
GTACCCT400.0294.995731
AGATCCT157.216302E-6294.995731
ATGGCCT255.275069E-10294.995731
ACCGCCT206.150549E-8294.995731
CTGAGAG157.216302E-6294.99573295
GATCCTA1250.0294.995732
ATTACCT206.150549E-8294.995731
GGTCCTA1550.0294.995732
GTCTCGT108.451072E-4294.99573295
CGCGCCT108.451072E-4294.995731
TCCTCGG157.216302E-6294.995734
GCGCCCT303.6379788E-12294.995731
CGCGAGG108.451072E-4294.995739
CCTCGGA108.451072E-4294.995735
CGTTCCT303.6379788E-12294.995731
Produced by FastQC (version 0.11.5)