FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81633
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1370.16782428674678132No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1370.16782428674678132No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1300.15924932319037644No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.15679933360283219No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.15067435963397155No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.15067435963397155No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.14944936484019944No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.1482243700464273No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.14699937525265516No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.1445493856651109No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.1445493856651109No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1433243908713388No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14087440128379455No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14087440128379455No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14087440128379455No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14087440128379455No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.14087440128379455No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.13964940649002242No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13842441169625028No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.13719941690247817No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.13719941690247817No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.13597442210870603No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.13597442210870603No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.13597442210870603No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13474942731493392No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13474942731493392No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13474942731493392No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13474942731493392No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.13352443252116178No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.13352443252116178No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.13352443252116178No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.13229943772738967No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.13229943772738967No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.13229943772738967No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.13229943772738967No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13107444293361753No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.13107444293361753No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.1298494481398454No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12862445334607328No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12862445334607328No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12739945855230114No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12739945855230114No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12739945855230114No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12739945855230114No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.12617446375852903No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1249494689647569No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12372447417098478No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12372447417098478No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12372447417098478No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12372447417098478No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12372447417098478No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.12249947937721264No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.12249947937721264No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.1212744845834405No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.1212744845834405No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.1212744845834405No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.1200494897896684No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.1200494897896684No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.1200494897896684No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11882449499589626No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11882449499589626No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11882449499589626No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11759950020212415No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
CGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11637450540835201No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1151495106145799No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1151495106145799No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1151495106145799No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1151495106145799No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1151495106145799No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11392451582080776No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11392451582080776No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11269952102703565No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11269952102703565No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11269952102703565No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11269952102703565No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11269952102703565No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11147452623326351No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11147452623326351No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11147452623326351No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
ATGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11024953143949137No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
ACCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10902453664571926No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10779954185194712No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10779954185194712No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10779954185194712No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10779954185194712No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10657454705817501No Hit
CAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10534955226440287No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10534955226440287No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10534955226440287No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10534955226440287No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10534955226440287No Hit
AAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10412455747063076No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10289956267685862No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10167456788308649No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10167456788308649No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10167456788308649No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10044957308931438No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10044957308931438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)