FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73417
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.14165656455589307No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.13620823514989716No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.13620823514989716No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.1253115763379054No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.12394949398640642No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.12394949398640642No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.12122532928340848No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.11986324693190951No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.11986324693190951No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.11850116458041052No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11713908222891155No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11713908222891155No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.11577699987741258No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.11577699987741258No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11441491752591361No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11305283517441464No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.11305283517441464No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11305283517441464No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11305283517441464No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11305283517441464No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.11169075282291567No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1103286704714167No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.1103286704714167No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1103286704714167No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1103286704714167No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.1103286704714167No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10896658811991773No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10760450576841876No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10760450576841876No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10760450576841876No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10760450576841876No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10760450576841876No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10624242341691979No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10624242341691979No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10624242341691979No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10624242341691979No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10488034106542082No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10488034106542082No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10488034106542082No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10488034106542082No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10488034106542082No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.10351825871392185No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10351825871392185No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10351825871392185No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10351825871392185No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10215617636242288No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10215617636242288No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10215617636242288No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10215617636242288No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10215617636242288No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1007940940109239No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1007940940109239No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1007940940109239No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1007940940109239No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1007940940109239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT350.0294.990481
TTGTCCT450.0294.990481
GCCTCCT108.449737E-4294.990481
ATCTCCT255.2568794E-10294.990481
CACACCT450.0294.990481
GTGCACA108.449737E-4294.99048295
TGACCTA900.0294.990482
TACAGGA108.449737E-4294.990487
CTCCCTA1000.0294.990482
CGCCCTA800.0294.990482
AAATCCT450.0294.990481
GATACCT108.449737E-4294.990481
GGACCTA1150.0294.990482
GTCGCCT255.2568794E-10294.990481
CCTGCCT108.449737E-4294.990481
ACCACCT350.0294.990481
GGACCCT255.2568794E-10294.990481
CACGCCT108.449737E-4294.990481
GGTCCCT255.2568794E-10294.990481
CGCTCCT108.449737E-4294.990481
Produced by FastQC (version 0.11.5)