FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68700
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA18352.671033478893741No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA17822.593886462882096No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15072.1935953420669576No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14502.1106259097525473No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11951.7394468704512371No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11001.6011644832605532No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10691.5560407569141195No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10531.5327510917030567No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10231.4890829694323144No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9921.4439592430858805No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9911.4425036390101893No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9881.438136826783115No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9721.4148471615720524No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9091.3231441048034933No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9041.3158660844250363No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8961.304221251819505No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8591.250363901018923No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8521.240174672489083No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8341.2139737991266375No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7911.1513828238719068No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6680.9723435225618632No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6610.9621542940320233No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6590.9592430858806406No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6550.9534206695778749No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6310.9184861717612809No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6220.9053857350800582No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6170.8981077147016011No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6140.8937409024745269No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6070.883551673944687No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5960.8675400291120815No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5950.86608442503639No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5910.8602620087336245No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5760.8384279475982532No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5690.8282387190684134No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5670.8253275109170306No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5660.8238719068413392No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5270.7671033478893741No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5270.7671033478893741No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4870.7088791848617176No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4740.6899563318777293No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4630.6739446870451238No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4540.6608442503639009No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4350.6331877729257642No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4130.6011644832605532No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4100.5967976710334789No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4000.5822416302765648No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3980.5793304221251819No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3850.5604075691411936No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3830.5574963609898107No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3670.5342066957787481No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3600.5240174672489083No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3590.5225618631732168No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3460.5036390101892285No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3430.49927219796215433No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3330.4847161572052402No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3290.47889374090247455No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3180.462882096069869No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3180.462882096069869No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3090.4497816593886462No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3080.44832605531295483No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2570.37409024745269287No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2520.3668122270742358No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2490.3624454148471616No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2420.3522561863173217No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2400.34934497816593885No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2290.33333333333333337No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.33333333333333337No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2280.3318777292576419No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2190.3187772925764192No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2160.314410480349345No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2110.3071324599708879No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2030.2954876273653566No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2020.2940320232896652No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1980.28820960698689957No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1970.2867540029112082No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1950.2838427947598253No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.2838427947598253No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1860.2707423580786026No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1740.25327510917030566No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1730.25181950509461426No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1640.23871906841339158No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.23289665211062593No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1420.2066957787481805No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1360.19796215429403202No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1320.19213973799126638No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1310.19068413391557495No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1300.18922852983988353No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1290.18777292576419213No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1260.1834061135371179No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1260.1834061135371179No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1250.1819505094614265No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1240.18049490538573507No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1240.18049490538573507No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1210.17612809315866085No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1170.1703056768558952No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1170.1703056768558952No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1160.16885007278020378No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1090.1586608442503639No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1090.1586608442503639No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1050.15283842794759825No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1030.14992721979621543No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1000.1455604075691412No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA990.14410480349344978No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA990.14410480349344978No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA970.14119359534206696No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA960.13973799126637554No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA950.13828238719068411No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG940.13682678311499272No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA910.13245997088791847No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA900.13100436681222707No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA890.12954876273653565No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA890.12954876273653565No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCA880.12809315866084425No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA860.12518195050946143No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA850.12372634643377002No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG850.12372634643377002No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA840.12227074235807861No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCA820.11935953420669579No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA810.11790393013100438No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.11790393013100438No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA800.11644832605531297No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG790.11499272197962153No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG790.11499272197962153No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA780.11353711790393012No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA780.11353711790393012No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA780.11353711790393012No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA770.11208151382823871No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA760.1106259097525473No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG750.10917030567685589No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA730.10625909752547306No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA710.10334788937409024No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA690.10043668122270742No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.10043668122270742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC66900.0295.01
TCGGGTA4650.0295.08
TGTTAGC206.142909E-8295.0295
AGTTCAC108.4477436E-4295.0295
AATTAGG108.4477436E-4295.0295
AATTAGC550.0295.0295
CGGGGTA3350.0294.999978
GACTACC26000.0294.432682
CTACCCG12500.0293.824
GGGGTTT10700.0293.621529
ACTACCG7550.0293.046363
ACCCGGG12550.0292.64946
TACTGGG6250.0292.639985
ACTACCA6150.0292.601623
GACTACT26900.0291.710022
ACTCGGG13250.0291.66046
TACTCGG13250.0291.66045
CTCGGGT13200.0291.647747
ACTACCC12550.0291.474123
CTACCGG7600.0291.11844
Produced by FastQC (version 0.11.5)