FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103706
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1160.1118546660752512No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1130.10896187298709814No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.10414055117350972No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1070.10317628681079205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCT550.0295.000031
GTCGCCT305.456968E-12295.01
GCGACCT450.0295.01
GCGCCCT255.275069E-10295.01
CGTTCCT450.0295.01
GACCCCT450.0295.01
TGCGCCT255.275069E-10295.01
GAGCCTA1200.0295.02
GGGCCTA1250.0295.02
CGTACCT450.0295.01
CCGACCT305.456968E-12295.01
TAGTCCT600.0295.01
GTGCCTA750.0295.02
CAACGGG108.453947E-4294.999976
GGTGCCT108.453947E-4294.999971
TTACGGG108.453947E-4294.999976
GGCGCCT108.453947E-4294.999971
GGTCTAC108.453947E-4294.999972
GATTCCT206.158007E-8294.999971
AGCCTAC5650.0292.389373
Produced by FastQC (version 0.11.5)