FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81572
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG890.10910606580689453No Hit
TTCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG840.10297651154808021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCT550.0295.000031
TCGCCCT500.0295.01
GTCGCCT157.2157727E-6295.01
GTCTACG108.450638E-4295.03
CGTTCCT157.2157727E-6295.01
GTGTCTA108.450638E-4295.01
ATCCCCT500.0295.01
AACGCCT350.0295.01
TTGCCTA650.0295.02
GACGCCT303.6379788E-12295.01
CGGTCCT400.0295.01
GCTACGG157.2157727E-6295.04
TTCGCCT255.275069E-10295.01
ACGAGGC108.450638E-4295.08
CCGCCTA950.0295.02
TAGTCCT303.6379788E-12295.01
GTTGCCT108.450638E-4295.01
ACCTACG21350.0291.545654
CCTACGG78300.0290.290565
TCCTACG22800.0289.82464
Produced by FastQC (version 0.11.5)