FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49196
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2800.0295.000033
CGGTCCT206.125265E-8295.01
CGTCCTA550.0295.02
CCGCCCT255.2386895E-10295.01
ACGACCT108.440437E-4295.01
GACGCCT157.1983577E-6294.999971
TCCTACG11450.0293.71184
CCTACGG44800.0291.70765
CTACGGG43950.0289.965886
TACGGGA43000.0289.16867
ACGGGAG43000.0289.16868
CCCTACG12400.0289.052434
GCCTACG8200.0287.804874
ACCTACG13200.0286.06064
GCCCTAC3400.0281.98533
GGTCCTA1100.0281.59092
CGGGAGG43200.0281.34269
GCCCCTA1000.0280.252
ACCCTAC3350.0277.388063
TTCCTAC3000.0275.333343
Produced by FastQC (version 0.11.5)