FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91067
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1700.18667574423226857No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1610.17679291071408962No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1480.16251770674338673No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1440.15812533629086278No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1420.15592915106460079No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15153678061207684No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15153678061207684No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.14824250277268386No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.14494822493329088No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.14494822493329088No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.14494822493329088No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.14385013232015986No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1300.14275203970702888No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.14165394709389792No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.1405558544807669No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.1405558544807669No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.13945776186763592No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.13945776186763592No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1250.13726157664137392No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1250.13726157664137392No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.13616348402824294No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.13506539141511195No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.13506539141511195No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13396729880198094No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.13396729880198094No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.13286920618884998No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.13286920618884998No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.13286920618884998No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13067302096258798No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13067302096258798No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13067302096258798No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.129574928349457No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.129574928349457No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.129574928349457No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.128476835736326No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.128476835736326No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.127378743123195No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.127378743123195No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.127378743123195No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.126280650510064No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.126280650510064No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.12518255789693303No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12408446528380204No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12408446528380204No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.12408446528380204No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12298637267067106No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12298637267067106No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12298637267067106No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.12188828005754006No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12079018744440906No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12079018744440906No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12079018744440906No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12079018744440906No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11969209483127807No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11969209483127807No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11969209483127807No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.11969209483127807No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11859400221814707No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11859400221814707No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.11859400221814707No Hit
AATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11639781699188509No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11639781699188509No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11639781699188509No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.11639781699188509No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11529972437875409No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11529972437875409No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.11529972437875409No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.11420163176562312No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11310353915249213No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.11310353915249213No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.11200544653936113No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.11200544653936113No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11090735392623013No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11090735392623013No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11090735392623013No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11090735392623013No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.10980926131309915No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10871116869996815No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10871116869996815No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10871116869996815No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.10871116869996815No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.10761307608683715No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10651498347370617No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10651498347370617No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.10651498347370617No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1054168908605752No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1054168908605752No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1054168908605752No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.1054168908605752No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1043187982474442No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1043187982474442No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1043187982474442No Hit
CCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1043187982474442No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.1043187982474442No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10322070563431321No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10322070563431321No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10322070563431321No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10322070563431321No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
GCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10212261302118221No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
ACGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
CTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10102452040805121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCT550.0295.000031
AAATCCT550.0295.000031
GTTCCTA550.0295.000032
CCCACCT550.0295.000031
GCGCCTA1100.0295.000032
ACCCCTA1100.0295.000032
TATTCCT550.0295.000031
ATTCCTA1550.0295.000032
AAACCTA2350.0295.02
GTTGATT255.275069E-10295.0295
TGGTCCT450.0295.01
AATCCTA1850.0295.02
TCCTCCT350.0295.01
GGGTCCT350.0295.01
CCTACGA255.275069E-10295.05
CCTACCT500.0295.01
CACACCT450.0295.01
TGCGCCT450.0295.01
GATACCT350.0295.01
AATTCCT350.0295.01
Produced by FastQC (version 0.11.5)