FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64155
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.11690437222352115No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10755202244563947No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC680.10599329748265918No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.1044345725196789No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGC670.1044345725196789No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.1044345725196789No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10287584755669862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA950.0295.000032
CTAGGGA350.0295.000036
TTCCCTA700.0295.000032
CTGGCCT350.0295.000031
CGCACCT350.0295.000031
TACTCCT350.0295.000031
AAGCCTA950.0295.000032
ATATCCT350.0295.000031
GCCCCTA700.0295.000032
ACAGCCT400.0295.01
AACGCCT450.0295.01
AATCCCT108.44644E-4295.01
ACGCCCT108.44644E-4295.01
GCCTCCT108.44644E-4295.01
GTCACCT255.2568794E-10295.01
ATCTCCT206.139817E-8295.01
CACACCT206.139817E-8295.01
CGCCCTA1000.0295.02
TTTCCTA2100.0295.02
TTCGCCT206.139817E-8295.01
Produced by FastQC (version 0.11.5)