FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89874
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.11237955359725839No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.10459087166477513No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10347820281727753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT350.0295.000031
CGCACCT350.0295.000031
TGATCCT600.0295.01
TTCTACG157.2182156E-6295.03
AGATCCT255.275069E-10295.01
ACGCCTA1050.0295.02
GAGTCCT255.275069E-10295.01
GATCCTA1900.0295.02
CGACCTA1450.0295.02
AGGACCT450.0295.01
GCGCCCT157.2182156E-6295.01
TCCTAGG157.2182156E-6295.04
TTCCCTA1900.0295.02
TACGCCT255.275069E-10295.01
GGGACCT255.275069E-10295.01
AACGCCT600.0295.01
TACCTAG157.2182156E-6295.03
CATACCT255.275069E-10295.01
CTCGCCT157.2182156E-6295.01
CCGACCT500.0295.01
Produced by FastQC (version 0.11.5)