FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88115
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA1100.0295.000032
CGAACCT303.6379788E-12295.01
GTACAGG157.217739E-6295.0295
CGCGCCT157.217739E-6295.01
GCGCCTA1200.0295.02
GCGCCCT157.217739E-6295.01
CGCCTAC4200.0295.03
ATCCCCT255.275069E-10295.01
CTCGCCT500.0295.01
CGTACCT255.275069E-10295.01
GAACCTA1200.0295.02
GATTCCT255.275069E-10295.01
ACGACCT303.6379788E-12295.01
CCCGCCT450.0295.01
GCATCCT500.0295.01
CTAGCCT157.217739E-6295.01
TTGTCCT400.0294.999971
CCTCGGA108.451791E-4294.999975
AGAACCT206.152732E-8294.999971
GGGCGGC108.451791E-4294.999979
Produced by FastQC (version 0.11.5)