FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83236
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
ACGCCTA1400.0295.000032
GGCGCCT350.0295.000031
GCGACCT500.0295.01
GCGCCCT255.275069E-10295.01
CGGGGCA157.2162984E-6295.09
CTCGGGA650.0295.06
CGAGCCT450.0295.01
GACGCCT255.275069E-10295.01
GATTCCT450.0295.01
GCGCGAA108.4509474E-4294.99997295
CTTTTCG108.4509474E-4294.999976
GCACCCT206.150731E-8294.999971
GTGTCGG800.0294.99997295
TCGACCT108.4509474E-4294.999971
TCCTACG22550.0293.03774
GCCTACG15750.0291.253974
ACCTACG21150.0290.81564
GTCCTAC5800.0289.91383
ACGGGAG75050.0289.89018
Produced by FastQC (version 0.11.5)