FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69639
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10051838768506154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCT550.0295.000031
ACCTCCT350.0295.000031
TGACCTA1100.0295.000032
AGACCCT350.0295.000031
CCCCCTA700.0295.000032
GAACCTA1100.0295.000032
TTCGCCT350.0295.000031
CTAATAC350.0295.00003295
TGATCCT206.143455E-8295.01
ACAACCT255.2568794E-10295.01
ATCTCCT450.0295.01
GTCGCCT157.2112343E-6295.01
CGAACCT303.6379788E-12295.01
TCCGCCT303.6379788E-12295.01
CCGGCCT108.4479805E-4295.01
TTGTCCT303.6379788E-12295.01
GATCCCT108.4479805E-4295.01
GTCTCCT255.2568794E-10295.01
CGACCTC108.4479805E-4295.02
AAGACCT157.2112343E-6295.01
Produced by FastQC (version 0.11.5)