FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66230
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA700.0295.000032
TTCCCTA1250.0295.000032
TATTCCT350.0295.000031
TCCACCT350.0295.000031
GCCCCTA650.0295.02
GTCGCCT157.2096427E-6295.01
TGCCCCT255.2568794E-10295.01
GTCTCCT206.141272E-8295.01
CACGCCT157.2096427E-6295.01
AGGACCT303.6379788E-12295.01
ACCCTTT108.447049E-4295.0295
TAGCCTA1000.0295.02
CGTTCCT206.141272E-8295.01
ACGGGGC157.2096427E-6295.08
ACGGGAC108.447049E-4295.08
GCACCCT157.2096427E-6295.01
CGCTCCT157.2096427E-6295.01
TGCGCCT255.2568794E-10295.01
GTTCGAG108.447049E-4295.0295
CTCGGGA206.141272E-8295.06
Produced by FastQC (version 0.11.5)