FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76654
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA700.0295.000032
TACGCCT350.0295.000031
TACTCCT350.0295.000031
TCTCCCT350.0295.000031
CTCTCCT350.0295.000031
TTACCCT350.0295.000031
TCGCCTA1100.0295.02
GTCGCCT255.2568794E-10295.01
TGCCCCT400.0295.01
AGATCCT255.2568794E-10295.01
ACGCCTA950.0295.02
GCGACCT108.449649E-4295.01
GTACAGG206.1476385E-8295.0295
TACCACG108.449649E-4295.03
CACGGGA400.0295.06
CTATCCT206.1476385E-8295.01
ATTACCT400.0295.01
CTCCCTA1100.0295.02
CGACCTA1250.0295.02
CACGCCT206.1476385E-8295.01
Produced by FastQC (version 0.11.5)