FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60786
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC950.1562859869048794No Hit
ACATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC920.15135063994998849No Hit
ATCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC910.14970552429835818No Hit
TAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.14477017734346725No Hit
ATAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC870.14312506169183692No Hit
TTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC870.14312506169183692No Hit
TTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC860.14147994604020664No Hit
AATTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC860.14147994604020664No Hit
TCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC850.1398348303885763No Hit
TCCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC830.1365445990853157No Hit
CTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC830.1365445990853157No Hit
TTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC810.13325436778205507No Hit
ATCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.13160925213042476No Hit
TCGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.13160925213042476No Hit
TACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.13160925213042476No Hit
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.13160925213042476No Hit
CATTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.13160925213042476No Hit
TCCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.12996413647879446No Hit
TCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.12996413647879446No Hit
TCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.12996413647879446No Hit
ACCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC780.12831902082716415No Hit
ATCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC770.12667390517553384No Hit
CTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.12502878952390353No Hit
GTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC750.12338367387227321No Hit
CTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC750.12338367387227321No Hit
ACACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC740.12173855822064292No Hit
ATTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.1200934425690126No Hit
TTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.1200934425690126No Hit
ATTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.1200934425690126No Hit
GTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.11844832691738229No Hit
TAAGCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.116803211265752No Hit
AACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.116803211265752No Hit
AAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.116803211265752No Hit
CCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.116803211265752No Hit
CCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.116803211265752No Hit
CAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
CTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
TTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
TACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
TCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
CAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
CCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
CTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
AACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.11515809561412167No Hit
AAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC690.11351297996249138No Hit
CAGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC690.11351297996249138No Hit
ATTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC690.11351297996249138No Hit
ACAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC690.11351297996249138No Hit
TAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11186786431086106No Hit
GTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11186786431086106No Hit
GTGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11186786431086106No Hit
ATGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11186786431086106No Hit
CTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11186786431086106No Hit
TCCGCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC670.11022274865923073No Hit
TGGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC670.11022274865923073No Hit
ACTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC670.11022274865923073No Hit
TTCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC670.11022274865923073No Hit
CCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC670.11022274865923073No Hit
ATATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC660.10857763300760044No Hit
TTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC660.10857763300760044No Hit
AAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC660.10857763300760044No Hit
GACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC660.10857763300760044No Hit
CCCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC660.10857763300760044No Hit
AGCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
TCTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
CACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
TACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
TTTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
AGATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
ACGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC650.10693251735597012No Hit
CCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
TTGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
GGCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
GCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
GAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
AACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10528740170433983No Hit
CGGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
AGCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
TCAGCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
CCGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
ACCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
GTGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC630.1036422860527095No Hit
CACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC620.10199717040107918No Hit
AATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC620.10199717040107918No Hit
GGATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC620.10199717040107918No Hit
CAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC620.10199717040107918No Hit
AGGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC620.10199717040107918No Hit
GTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
GGGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
CTGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
GGGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
TAGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
CACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit
ATAGCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC610.10035205474944889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCT350.0295.000031
ATCGCCT350.0295.000031
TCGCCTA800.0295.02
GTCGCCT206.137088E-8295.01
AGATCCT157.2067232E-6295.01
CGAACCT206.137088E-8295.01
GCGACCT108.445339E-4295.01
AGCCCTA600.0295.02
GATCCTA850.0295.02
CTCCCTA750.0295.02
TTGTCCT450.0295.01
CGACCTA750.0295.02
GGATCCT303.6379788E-12295.01
CGACCCT157.2067232E-6295.01
GCGCCCT108.445339E-4295.01
CGTTCCT206.137088E-8295.01
GACCCCT303.6379788E-12295.01
ACGGGAC108.445339E-4295.08
CCGTCCT206.137088E-8295.01
GTTACCT157.2067232E-6295.01
Produced by FastQC (version 0.11.5)