FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61820
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18703.0249110320284696No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA18352.968295050145584No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14402.329343254610159No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13962.2581688773859594No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13892.2468456810093818No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13872.243610482044646No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13162.1287609187965058No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12902.0867033322549338No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11761.9022969912649628No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11671.8877385959236492No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11551.8683274021352312No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10731.7356842445810416No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8771.4186347460368813No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8661.4008411517308315No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6861.1096732449045617No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6090.9851180847622129No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6080.9835004852798448No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6070.9818828857974766No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5970.9657068909737949No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5870.9495308961501133No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5370.8686509220317049No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5360.8670333225493367No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5330.8621805241022323No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5000.8087997411840828No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4960.8023293432546102No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4840.7829181494661922No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4730.7651245551601423No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4710.761889356195406No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4630.7489485603364607No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4560.7376253639598835No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4500.7279197670656745No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4450.7198317696538338No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4310.6971853769006794No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4230.6842445810417341No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4150.6713037851827888No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4150.6713037851827888No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4080.6599805888062116No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4080.6599805888062116No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4050.6551277903591071No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3940.6373341960530573No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3910.6324813976059528No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3820.6179230022646393No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3750.6065998058880622No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3690.5968942089938531No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3690.5968942089938531No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3540.5726302167583307No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3530.5710126172759624No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3280.5305726302167584No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3220.5208670333225494No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3200.517631834357813No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3190.5160142348754448No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3160.5111614364283403No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3150.5095438369459722No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3140.507926237463604No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3100.5014558395341313No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2930.4739566483338726No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2800.4529278550630864No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2730.4416046586865092No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2720.4399870592041411No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2450.3963118731802006No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2210.35748948560336463No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2140.34616628922678744No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2090.33807829181494664No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2040.3299902944031058No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1960.3170494985441604No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1930.31219670009705597No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1890.30572630216758334No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1870.30249110320284694No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1770.28631510837916535No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1760.2846975088967972No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1750.28307990941442895No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1750.28307990941442895No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1700.27499191200258816No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.27499191200258816No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1670.27013911355548365No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1620.26205111614364285No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1600.2588159171789065No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1570.253963118731802No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.253963118731802No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1570.253963118731802No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1530.24749272080232934No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1490.2410223228728567No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1470.23778712390812035No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.22808152701391135No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1400.2264639275315432No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1340.21675833063733418No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1300.21028793270786153No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1280.20705273374312522No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1280.20705273374312522No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1270.20543513426075702No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1230.19896473633128436No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1230.19896473633128436No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1230.19896473633128436No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1200.19411193788417988No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1190.1924943384018117No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1190.1924943384018117No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1180.19087673891944354No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1090.17631834357813006No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1080.1747007440957619No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1080.1747007440957619No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1050.1698479456486574No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1000.16175994823681655No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA980.1585247492720802No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA980.1585247492720802No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA960.1552895503073439No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA940.15205435134260759No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA940.15205435134260759No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA940.15205435134260759No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA910.14720155289550307No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA890.14396635393076673No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA880.1423487544483986No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA880.1423487544483986No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA880.1423487544483986No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA870.14073115496603042No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA850.13749595600129408No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA850.13749595600129408No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.13426075703655774No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA820.1326431575541896No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA820.1326431575541896No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA810.13102555807182142No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA800.12940795858945325No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.12617275962471694No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA780.12617275962471694No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA780.12617275962471694No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA760.1229375606599806No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA760.1229375606599806No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA730.11808476221287609No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA720.11646716273050793No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA720.11646716273050793No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA720.11646716273050793No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA690.11161436428340343No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA680.10999676480103528No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA670.10837916531866709No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA670.10837916531866709No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA660.10676156583629894No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA660.10676156583629894No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA650.10514396635393077No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA650.10514396635393077No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA630.10190876738919444No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA620.10029116790682628No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA620.10029116790682628No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA620.10029116790682628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)