FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70088
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.10130122132176692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA700.0295.000032
TCACCCT350.0295.000031
GGTCCCT350.0295.000031
TGAACCT350.0295.000031
CTCGCCT350.0295.000031
CCGCCCT350.0295.000031
CACCCTA1100.0295.000032
GTGCCTA350.0295.000032
AGCACCT400.0295.01
TCGCCTA1300.0295.02
GTTGATT157.2114326E-6295.0295
GTGCATC157.2114326E-6295.0295
TCGCCCT303.6379788E-12295.01
GCCCCCT255.2568794E-10295.01
GATACCT157.2114326E-6295.01
GTCGCCT157.2114326E-6295.01
CAAACCT450.0295.01
AGATCCT206.1438186E-8295.01
TGCCAAG108.4480975E-4295.0295
AGCCCTA650.0295.02
Produced by FastQC (version 0.11.5)