FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76124
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC960.1261100310020493No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC910.11954180022069255No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC900.11822815406442119No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC850.11165992328306448No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.10903263097052178No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.10903263097052178No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.10771898481425042No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10640533865797909No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10640533865797909No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10640533865797909No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.10640533865797909No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.1037780463454364No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.1037780463454364No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.1037780463454364No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.10246440018916504No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.10246440018916504No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.10246440018916504No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.1011507540328937No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.1011507540328937No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.1011507540328937No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.1011507540328937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT350.0295.000031
GATACCT350.0295.000031
ATCTCCT350.0295.000031
ATGGCCT350.0295.000031
CGCTCCT350.0295.000031
TTGACCT350.0295.000031
CCGCCTA700.0295.000032
CCCGCCT350.0295.000031
GTGCCTA700.0295.000032
GTTGATT303.6379788E-12295.0295
GCCCCTA1050.0295.02
GTCGCCT206.147275E-8295.01
AGTCCCT157.213879E-6295.01
CCCCTAC4800.0295.03
AGATCCT303.6379788E-12295.01
CGAACCT206.147275E-8295.01
GCGACCT500.0295.01
TCACCCT255.2568794E-10295.01
CAGCCTA1150.0295.02
CCTAGGG500.0295.05
Produced by FastQC (version 0.11.5)