FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56530
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT303.6379788E-12295.01
GCGACCT350.0295.01
TAGACCT303.6379788E-12295.01
CGACCTA1200.0295.02
GCGCCTA600.0295.02
CCTACCG108.443777E-4295.05
CGTTCCT108.443777E-4295.01
CGGGGGT108.443777E-4295.09
GTTACCT157.2040566E-6295.01
GTAGCCT206.1332685E-8295.01
CCCCCCT303.6379788E-12295.01
CTCGCCT157.2040566E-6295.01
GACGCCT350.0295.01
GGCGCCT206.1332685E-8295.01
GGACCCT157.2040566E-6295.01
TCCTACG13800.0292.862344
CCCTACG13200.0291.647744
CCTACGG52150.0290.474585
GTCCTAC3250.0290.461553
CTACGGG50800.0290.063966
Produced by FastQC (version 0.11.5)