FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70030
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10995287733828359No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10281308010852493No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10138512066257319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA1250.0295.000032
TCGCCTA650.0295.02
AGATCCT206.1438186E-8295.01
ACGCCTA850.0295.02
GCGACCT350.0295.01
CAACGGG108.4480847E-4295.06
AGCCCTA550.0295.02
GGTCCTA1000.0295.02
TGAACCT255.2568794E-10295.01
CGCTCCT206.1438186E-8295.01
TATTATC400.0295.0295
CTCGCCT108.4480847E-4295.01
CCGACCT450.0295.01
TGGGCCT450.0295.01
CGTCCTA1100.0295.02
TTCGCCT255.2568794E-10295.01
CGATCCT206.1438186E-8295.01
TCGACCT206.1438186E-8295.01
CGCCCTA950.0295.02
TAGTCCT500.0295.01
Produced by FastQC (version 0.11.5)