FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77239
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.13464700475148564No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.12558422558552026No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.12040549463354004No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.12040549463354004No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11522676368155983No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11522676368155983No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11522676368155983No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11393208094356477No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11263739820556974No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11263739820556974No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.11134271546757468No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.11004803272957962No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10875334999158456No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.1074586672535895No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.1074586672535895No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.10616398451559445No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.10616398451559445No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.10616398451559445No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.1048693017775994No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.1022799363016093No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.1022799363016093No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.1022799363016093No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.1022799363016093No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.1022799363016093No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10098525356361424No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10098525356361424No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10098525356361424No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10098525356361424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA700.0295.02
GTCGCCT206.1478204E-8295.01
GTACCCT303.6379788E-12295.01
TGCCCCT206.1478204E-8295.01
AGATCCT157.214283E-6295.01
TAAAGCC108.449767E-4295.0295
CGGCCTA800.0295.02
GAGTCCT255.2568794E-10295.01
TGGACCT157.214283E-6295.01
CCGGCCT350.0295.01
ATTACCT500.0295.01
CACGCCT303.6379788E-12295.01
AGGACCT303.6379788E-12295.01
CGCGCCT206.1478204E-8295.01
GGATCCT255.2568794E-10295.01
CGACCCT255.2568794E-10295.01
GCGCCTA750.0295.02
CCTACGC157.214283E-6295.05
GCGCCCT157.214283E-6295.01
CTAAGGG108.449767E-4295.06
Produced by FastQC (version 0.11.5)