FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80688
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT350.0295.000031
CGCCCTA850.0295.000032
TCGCCTA650.0295.02
GCCCCTA1650.0295.02
CGAACCT400.0295.01
GAGTCCT303.6379788E-12295.01
GGTCCTA1850.0295.02
GTCTCGT157.215478E-6295.0295
CGACCTA1200.0295.02
AAGCCTA800.0295.02
TACGCCT450.0295.01
TGCGCCT255.275069E-10295.01
AGCGCCT255.275069E-10295.01
AGCGAGG108.450465E-4295.0295
AATTCCT108.450465E-4295.01
GGCGCCT255.275069E-10295.01
TTCGCCT157.215478E-6295.01
CGATCCT303.6379788E-12295.01
CCCTACG18250.0294.191774
CCCCTAC4750.0291.894743
Produced by FastQC (version 0.11.5)